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Live cell imaging by conventional and spinning disk confocal microscopy

 

 

Mouse Cardiomyocyte


Depolarization evoked calcium waves in mouse cardiomyocytes (Bio-Rad confocal laser scanning microscope; calcium sensitive fluorescent dye Fluo-3 AM; Wernicke_D_AJP_2004.pdf

 

 
   
 

 

Mouse Oocytes


Carbachol, a non-hydrolyzable acetylcholine analogue, induces calcium oscillations in mouse oocytes via activation of muscarinic receptors and subsequent stimulation of the PLC-IP3 pathway and IP3-mediated calcium release from the ER (Perkin Elmer, UltraView LCI laser spinning disk confocal microscope; calcium sensitive fluorescent dye Fluo-4 AM).

 

 
     
 

 

C.elegans Pharynx


Calcium waves in C.elegans pharyngeal muscle syncytium (fluorescence digital microscope camera JenOptik ProgRes MF; genetically encoded calcium indicator GCamP3).

 

 
     
 

 

Rat Arterial Smooth Muscle Cell


Spontaneous local calcium release (calcium spark) in a rat tibial artery smooth muscle cell (Perkin Elmer, UltraView LCI laser spinning disk confocal microscope; calcium sensitive fluorescent dye Fluo-3 AM; Essin_K_JBB_2009.pdf, Fig 1.).

 

 
     
 

 

Human Blood Neutrophil

fMLP, a proinflammatory N-formylated peptide released by bacteria, induces intracellular calcium rise in a human blood neutrophil (Perkin Elmer, UltraView LCI laser spinning disk confocal microscope; calcium sensitive fluorescent dye Fluo-4 AM; Essin_K_AJP_2007.pdf, Fig 3.).

 
     
 

 

Mouse Rod Bipolar Cell
Depolorization-induced increase in intra-bouton calcium concentration in a mouse rod bipolar cell. Cell soma and synaptic boutons are seen at the left and right sides of the up video, respectively (Andor iXON camera attached to a light microscpe; calcium sensitive fluorescent dye Fluo-8 loaded via a somatic patch pipette). The time course of intra-bouton calcium changes is present in the bottom video Mouse_Retinal_Slices_Method.zip

Kirill Esin, essine_kv@yahoo.com Back