Carbachol, a non-hydrolyzable acetylcholine analogue, induces calcium oscillations in mouse oocytes via activation of muscarinic receptors and subsequent stimulation of the PLC-IP3 pathway and IP3-mediated calcium release from the ER (Perkin Elmer, UltraView LCI laser spinning disk confocal microscope; calcium sensitive fluorescent dye Fluo-4 AM).
C.elegans Pharynx
Calcium waves in C.elegans pharyngeal muscle syncytium (fluorescence digital microscope camera JenOptik ProgRes MF; genetically encoded calcium indicator GCamP3).
Rat Arterial Smooth Muscle Cell
Spontaneous local calcium release (calcium spark) in a rat tibial artery smooth muscle cell (Perkin Elmer, UltraView LCI laser spinning disk confocal microscope; calcium sensitive fluorescent dye Fluo-3 AM;
Essin_K_JBB_2009.pdf, Fig 1.).
Human Blood Neutrophil
fMLP, a proinflammatory N-formylated peptide released by bacteria, induces intracellular calcium rise in a human blood neutrophil (Perkin Elmer, UltraView LCI laser spinning disk confocal microscope; calcium sensitive fluorescent dye Fluo-4 AM;
Essin_K_AJP_2007.pdf, Fig 3.).
Mouse Rod Bipolar Cell
Depolorization-induced increase in intra-bouton calcium concentration in a mouse rod bipolar cell.
Cell soma and synaptic boutons are seen at the left and right sides of the up video, respectively
(Andor iXON camera attached to a light microscpe; calcium sensitive fluorescent dye Fluo-8 loaded via a somatic patch pipette).
The time course of intra-bouton calcium changes is present in the bottom video
Mouse_Retinal_Slices_Method.zip